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pet22b backbone  (Addgene inc)


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    Addgene inc pet22b backbone
    Pet22b Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet22b backbone/product/Addgene inc
    Average 91 stars, based on 26 article reviews
    pet22b backbone - by Bioz Stars, 2026-05
    91/100 stars

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    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Journal: Frontiers in Microbiology

    Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies

    doi: 10.3389/fmicb.2022.877813

    Figure Lengend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Article Snippet: The cDNA sequence coding for SCoV-2 PLpro was codon-optimised for bacterial expression and was custom-synthesised in pET22b vector backbone by GeneScript, USA.

    Techniques: Plasmid Preparation, Expressing, Synthesized, Purification